When designing a dynamic metabolic controller, why is it necessary to incorporate a biosensor with a high binding affinity for the target intermediate rather than the final product?

In metabolic engineering, a dynamic controller is a regulatory circuit that adjusts enzyme activity based on the concentration of a specific molecule to prevent metabolic imbalances. A biosensor is a protein or RNA molecule that detects the presence of a specific target and triggers a genetic response, such as activating or repressing an enzyme. High binding affinity means the biosensor forms a very strong and stable connection with its target even when that target is present at low concentrations. It is necess....