What is the recommended action if a chemical indicator fails to change color during a steam sterilization cycle?

If a chemical indicator fails to change color during a steam sterilization cycle, the load is considered non-sterile and should not be used. The first step is to quarantine the load and prevent its use. Next, the sterilizer should be taken out of service and the sterilization cycle should be thoroughly investigated. This investigation includes reviewing the sterilizer's cycle parameters, such as temperature and pressure readings, to identify any deviations from the established sterilization parameters. Check the sterilizer for mechanical malfunctions, such as leaks or faulty valves, that may have affected the cycle. The chemical indicator itself should also be examined to ensure it was not expired or damaged. Another chemical indicator from the same lot number should be tested to rule out a defective indicator. If the investigation reveals a clear cause for the failure, such as a mechanical malfunction, the sterilizer should be repaired and revalidated before being returned to service. If the cause of the failure is not immediately apparent, a biological indicator test should be performed to assess the sterilizer's ability to kill microorganisms. A failed chemical indicator indicates a potential sterilization failure and requires immediate action to prevent the use of non-sterile items.